The translation of microscopy with ultraviolet surface excitation (MUSE) into a high school science classroom is investigated with the goal of providing a suitable new modality to enhance life science education. A key part of this effort is the development of laboratory exercises that can integrate the advanced capabilities of MUSE into a classroom setting. MUSE utilizes the unique property of ultraviolet light at wavelengths between 250 and 285 nm to propagate about 10 μm into tissues, thus illuminating only the top cell layer. This illumination is provided by a low-power UV LED source, which enables one to cost-efficiently implement this method into the educational environment. MUSE in education can eliminate the need for premade microscope slides and provide a far more engaging and rewarding experience for students.
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