Usually, the response of biological cells to electrical excitations is given by its dipolar part. In addition to that, the shape models of the cells are restrained, in general, to ellipsoidal shape and, in particular, to spherical or spheroidal shapes. However, the electric/dielectric response of the live cells may contain higher multipolar components whose origins comes from the cell shape (other than the ellipsoidal one) and from the multipolar parts of the applied field. In the present work we consider the response of arbitrarily-shaped cells beyond dipolar approximation. We use a boundary integral equation (BIE) method that intrinsically includes all multipolar orders of the response. For small field non-uniformity, we show that although a cell of a certain shape has a relatively large higher order multipolar term in its dielectric response, the dielectrophoretic force is accurately modeled with the dipolar response of a close spherical cell.
Digital holographic microscopy is a technique which enables real time monitoring of fast phenomena by using high
speed sensors of video cameras. Using this advantage, we obtain holographic images of flow in microcavities,
employing a CMOS video camera sensor with acquisition rate of 10 000fps. The corresponding reconstructed 3D image
for different flow conditions is obtained from a single hologram using simulations based on the Fresnel approximation.
We develop an automated image processing procedure in order to obtain quantitative information about the dynamic
contact angle evolution, the shape and velocity of an approximately 300μm wide portion from the water-air meniscus
interface in different microscopic cavity geometries.
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