A novel on-chip chemiluminescence biosensor is developed to assess cleanliness of the solid surfaces. The sensor is based on ATP-mediated chemiluminescence detection. It provides high-sensitivity detection of surface contamination due to improved photon collection efficiency. The results show the dependence of the bioluminescence enhancement on ATP concentration. We report the limit of detection of the biosensor. We demonstrate the use of the biosensor for real sample analysis to assess surface contamination of the laboratory and office equipment. This suggests the use of the biosensor in practical applications such as the food processing industry, laboratory environments, and health care settings.
SCOBY is a biomaterial derived from a symbiotic culture of bacteria and yeast. Its structure is characterized by intercalated nanocellulose fibers. There has been increased interest in using this bacterial nanocellulose to medical applications and in biosensors. For successful applications, their mechanical, optical, and electrical properties need to be characterized and improved. We report experimental results on the optical absorption, emission, and scattering properties of SCOBY and derivations of SCOBY. In addition, we demonstrate improved electrical conductivity by the addition of PEDOT:PSS and AgNPs into the nanocellulose matrix, leading to the possibility of SERS and luminescence emission for nanoscale biosensing applications.
A complex instrument is ordinarily used in electrochemiluminescence (ECL) biosensors to monitor the emitted light from the chemiluminescence reaction. As a result, these biosensors may not be suitable for point-of-need (PON) testing, which is critical in healthcare diagnostics. Microfluidic and luminol-based ECL systems were integrated on a CMOS chip to create a miniaturized ECL sensor for PON applications in this study. The findings demonstrated that this novel lab-on-a-chip system could detect uric acid levels as an essential biomarker for diagnosing gout disease in urine and saliva at levels lower than the physiological range. The device's repeatability, reproducibility, and selectivity were also investigated.
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