Spontaneous Raman flow cytometers and cell sorters typically have cell or particle throughputs less than 5 event/s due to long signal integration times. Here we report an up to 10x increase in throughput by coupling constant illumination along the flow path with time delay integration, a technique that counteracts sample motion by matching it to the charge transfer velocity of a CCD device. This allows longer signal integration times while also multiplexing acquisition along the flow path. We demonstrate high spectral bandwidth (600 – 3200 cm-1) Raman acquisitions from flowing particles and mammalian cells at throughputs up to 50 events/s.
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