Paper
7 October 2020 Miniaturized multi-contrast quantitative phase imaging microscope
Author Affiliations +
Proceedings Volume 11571, Optics Frontier Online 2020: Optics Imaging and Display; 1157119 (2020) https://doi.org/10.1117/12.2581013
Event: Optics Frontiers Online 2020: Optics Imaging and Display (OFO-1), 2020, Shanghai, China
Abstract
We present a miniaturized microscopic imaging system to achieve multi-contrast label-free imaging. In our imaging system, a highly integrated optical system using a miniaturization lens with fixed focal length replaces the complex optical path of traditional microscope systems, significantly reducing the size of the microscope to 14*16.5*20 cm3 . A programmable full-color light emitting diode (LED), which is controlled by independently designed operating software, is used to illuminate the sample in different illumination patterns. In addition, we developed a QT-based operating software to implement the synchronous control of the hardware system to achieve various label-free imaging approaches, including bright field, dark field, rainbow dark field, Rheinberg, differential phase contrast, and quantitative phase imaging. All the microscopic imaging approaches and system parameters can be controlled by the software without any hardware modification. In addition, some functions of the cell analysis are also added to our system, which can realize cell counter, 3D information measurement. We demonstrated our miniaturized system and its imaging results through experiments, which showed that our system is simple and fast to operate, providing more visual imaging results and high-resolution 3D information for samples. The experiment on living cell demonstrated that our miniaturized system can be built into an incubator for imaging and analysis of living cells in an environment suitable for cell growth.
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yao Fan, Junyi Ding, Jiasong Sun, Qian Chen, and Chao Zuo "Miniaturized multi-contrast quantitative phase imaging microscope", Proc. SPIE 11571, Optics Frontier Online 2020: Optics Imaging and Display, 1157119 (7 October 2020); https://doi.org/10.1117/12.2581013
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KEYWORDS
Imaging systems

Microscopes

Light emitting diodes

Cameras

Visualization

Control systems

3D metrology

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